Exploring Mitochondrial Dynamics with Multiphoton Intravital Microscopy

Advancements in Microscopy Technology

Recent developments in multiphoton intravital microscopy have opened new avenues for studying mitochondrial dynamics in small animals. Researchers have enhanced a conventional two-photon microscope by equipping it with 3D-printed holders. These holders stabilize the tissue surface, allowing for more precise observations.

Schematic representation of suction-based stab technique

This innovative approach significantly improves the accuracy of imaging and facilitates long-term studies of mitochondrial behavior in living organisms. By capturing real-time data, scientists can better understand the intricate processes that govern cellular energy production and metabolic functions.